Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 70
... density gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer ...
... density gradient which is at all points greater than the negative density gradient due to solute at the leading edge of the zone . In the method of Rosenbloom and Schumaker ( 1963 ) a density gradient is formed in the solvent layer ...
Page 73
... density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical protein ...
... density gradient of the protein solute is proportional to the initial protein concentration and is greatest during the first minutes of the run . After 15 minutes the maximum negative density gradient for the hypothetical protein ...
Page 84
... gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the redistribution of salt under the influence of the centrifugal ...
... gradient - forming salt alone . 4. The Different Density Gradients of Importance in Density Gradient Experiments a . The Composition Density Gradient . This is due to the redistribution of salt under the influence of the centrifugal ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone