Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 72
... diffusion coefficient of the protein . Figure 27 , taken from Vinograd and Bruner ( 1966b ) , shows how the concentra- tion of the zone maximum changes with time for solutes with various diffusion coefficients . It is apparent that for ...
... diffusion coefficient of the protein . Figure 27 , taken from Vinograd and Bruner ( 1966b ) , shows how the concentra- tion of the zone maximum changes with time for solutes with various diffusion coefficients . It is apparent that for ...
Page 74
... diffusion gradients as a function of both time and distance from the lamella - solution interface , for the diffusion of 10 μl of water into 1 M NaCl , 1 M CsCl or 95 % D2O , and 0.5 M KCl , in a 12 - mm center- piece . One molar CSCI ...
... diffusion gradients as a function of both time and distance from the lamella - solution interface , for the diffusion of 10 μl of water into 1 M NaCl , 1 M CsCl or 95 % D2O , and 0.5 M KCl , in a 12 - mm center- piece . One molar CSCI ...
Page 131
... diffusion ( fkT / D ° , where Do is the translational diffusion coefficient extrapolated to infinite dilution and k is Boltzmann's constant ) or sedimentation velocity ( ƒ = M ( 1 − ūp ) / Nso , where p is the density of solvent and so ...
... diffusion ( fkT / D ° , where Do is the translational diffusion coefficient extrapolated to infinite dilution and k is Boltzmann's constant ) or sedimentation velocity ( ƒ = M ( 1 − ūp ) / Nso , where p is the density of solvent and so ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone