Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 16
... effect for a 1 % protein solution . Higher protein concentrations require proportionately higher salt concentrations . Extrapolation to zero protein concentration at constant concentration of added electrolyte will eliminate the effect ...
... effect for a 1 % protein solution . Higher protein concentrations require proportionately higher salt concentrations . Extrapolation to zero protein concentration at constant concentration of added electrolyte will eliminate the effect ...
Page 286
... effect of a paramagnetic current opposing the local diamagnetic cur- rent . In contrast to the isotropic diamagnetic effect , the paramagnetic circulations are anisotropic and therefore depend on the orientation of the molecule with ...
... effect of a paramagnetic current opposing the local diamagnetic cur- rent . In contrast to the isotropic diamagnetic effect , the paramagnetic circulations are anisotropic and therefore depend on the orientation of the molecule with ...
Page 482
... effects of , 135 , 137 DNA , see Nucleic acids DNP - lysine , antibody binding to , 328 Donnan effect , 175 , 374 DSS , see Sodium 2,2 - dimethyl - 2- sialopentane - 5 - sulfonate Dust , in light scattering effect of , 159 , 194 removal ...
... effects of , 135 , 137 DNA , see Nucleic acids DNP - lysine , antibody binding to , 328 Donnan effect , 175 , 374 DSS , see Sodium 2,2 - dimethyl - 2- sialopentane - 5 - sulfonate Dust , in light scattering effect of , 159 , 194 removal ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone