Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 130
... estimate of the axial ratio ( Yang , 1961a ) . This treatment has been criticized by Scheraga and Mandelkern ( 1953 ) on various grounds , including the assumption of the equality of v and v , which is valid only for the case of an ...
... estimate of the axial ratio ( Yang , 1961a ) . This treatment has been criticized by Scheraga and Mandelkern ( 1953 ) on various grounds , including the assumption of the equality of v and v , which is valid only for the case of an ...
Page 244
... estimating visually when the best fit is obtained . The method may be use- ful for preliminary studies but there are two serious objections to its use . First , the estimation of best fit is subjective , and second , one can never be ...
... estimating visually when the best fit is obtained . The method may be use- ful for preliminary studies but there are two serious objections to its use . First , the estimation of best fit is subjective , and second , one can never be ...
Page 315
... estimated 90 % helical content . In both studies the helicity of the PBLG samples was estimated from the CaH resonances . Markley et al . ( 1967 ) used the observed chemical shifts ( 8obs ) , of the single resonance to calculate the ...
... estimated 90 % helical content . In both studies the helicity of the PBLG samples was estimated from the CaH resonances . Markley et al . ( 1967 ) used the observed chemical shifts ( 8obs ) , of the single resonance to calculate the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone