Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 41
... expression ( Schachman , 1959 , p . 86 ) Xa 8 = - ln 2+ ( 1-3 ) ] Cu Xa Co Xp ( 25 ) where x is the distance of the upper meniscus from the center of rotation and co is the initial concentration of solution in the cell . Sedimentation ...
... expression ( Schachman , 1959 , p . 86 ) Xa 8 = - ln 2+ ( 1-3 ) ] Cu Xa Co Xp ( 25 ) where x is the distance of the upper meniscus from the center of rotation and co is the initial concentration of solution in the cell . Sedimentation ...
Page 247
... expression = g 1 - sin2 ē ( 21 ) and the semivertical angle ( 0 % ) of a uniformly populated cone ( Trotter and Brown , 1956 ; Sandemann and Keller , 1956 ) , which is related to g by the expression . g = cos 00 ( 1 + cos 0。) These ...
... expression = g 1 - sin2 ē ( 21 ) and the semivertical angle ( 0 % ) of a uniformly populated cone ( Trotter and Brown , 1956 ; Sandemann and Keller , 1956 ) , which is related to g by the expression . g = cos 00 ( 1 + cos 0。) These ...
Page 382
... expression pH + log [ v / ( n - D ) ] = pKa - 0.868 wŻ ( 2 ) Here Ž is the average net charge borne by the protein , 0.868 represents 22.303 , and w is an electrostatic work . factor calculated according to the Debye - Hückel theory ...
... expression pH + log [ v / ( n - D ) ] = pKa - 0.868 wŻ ( 2 ) Here Ž is the average net charge borne by the protein , 0.868 represents 22.303 , and w is an electrostatic work . factor calculated according to the Debye - Hückel theory ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone