Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 29
... fraction assayed for the solute . The simplest way to achieve this is to pierce the base of the sample tube with a fine syringe needle and collect the solution with a fraction collector equipped with a drop counter . See for example ...
... fraction assayed for the solute . The simplest way to achieve this is to pierce the base of the sample tube with a fine syringe needle and collect the solution with a fraction collector equipped with a drop counter . See for example ...
Page 228
... fraction r of uniformly absorbing material and a fraction ( 1r ) of clear space ( Jones , 1952 ) . If accurate measurements are to be made , the specimen should be op- tically homogeneous as well as having a uniform thickness . In the ...
... fraction r of uniformly absorbing material and a fraction ( 1r ) of clear space ( Jones , 1952 ) . If accurate measurements are to be made , the specimen should be op- tically homogeneous as well as having a uniform thickness . In the ...
Page 249
... fraction of oriented chains ( b ) 0.5 1.0 Minimum fraction of oriented chains FIG . 16. The relation between dichroic ratio and the minimum value of the fraction of oriented chains g ( after Fraser , 1960 ) . ( Reproduced with ...
... fraction of oriented chains ( b ) 0.5 1.0 Minimum fraction of oriented chains FIG . 16. The relation between dichroic ratio and the minimum value of the fraction of oriented chains g ( after Fraser , 1960 ) . ( Reproduced with ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone