Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 48
... known levels using a precision syringe or microm- eter syringe . This is necessary to ensure that the solution column is always very slightly shorter than the solvent column as shown in Fig . 16 , so that the complete solution column is ...
... known levels using a precision syringe or microm- eter syringe . This is necessary to ensure that the solution column is always very slightly shorter than the solvent column as shown in Fig . 16 , so that the complete solution column is ...
Page 67
... known , as is c , the equations can be solved for c , and c2 and the equilibrium constant K = c2 / ( c1 ) 2 can be calculated . For a system in which each species behaves as if it were ideal , the same equilibrium constant should be ...
... known , as is c , the equations can be solved for c , and c2 and the equilibrium constant K = c2 / ( c1 ) 2 can be calculated . For a system in which each species behaves as if it were ideal , the same equilibrium constant should be ...
Page 111
... known values of T and w ; the constants of the viscometer can be determined by accurate measurement or by calibration with liquids of known viscosity . Equations ( 17 ) and ( 18 ) allow the calculation of and G at any value of the ...
... known values of T and w ; the constants of the viscometer can be determined by accurate measurement or by calibration with liquids of known viscosity . Equations ( 17 ) and ( 18 ) allow the calculation of and G at any value of the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone