Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 335
... lysozyme ( Dahlquist and Raftery , 1968b ) . The most recent study ( Raftery et al . , 1970 ) examines the binding of di- and trisaccharides and their methyl glycosides with lysozyme . It is demonstrated that NAG binds to only one of ...
... lysozyme ( Dahlquist and Raftery , 1968b ) . The most recent study ( Raftery et al . , 1970 ) examines the binding of di- and trisaccharides and their methyl glycosides with lysozyme . It is demonstrated that NAG binds to only one of ...
Page 349
... Lysozymes Early studies of lysozyme established the main features of the PMR spectrum , and showed that the changes observed in denatured states were qualitatively similar to those described for RNase ( Kowalsky , 1962 ; Mandel , 1964 ...
... Lysozymes Early studies of lysozyme established the main features of the PMR spectrum , and showed that the changes observed in denatured states were qualitatively similar to those described for RNase ( Kowalsky , 1962 ; Mandel , 1964 ...
Page 354
... lysozyme the a - helical regions are distorted from the classic Pauling helix ( NĂ©methy et al . , 1967 ; Leach et al . , 1968 ) . Any of these effects may contribute to the changes observed in the CaH region . Of particular note is an ...
... lysozyme the a - helical regions are distorted from the classic Pauling helix ( NĂ©methy et al . , 1967 ; Leach et al . , 1968 ) . Any of these effects may contribute to the changes observed in the CaH region . Of particular note is an ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone