Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 167
... medium composed of two or more solvent components present in such ratios that protein - solvent interactions may make important contributions . This is desirable under three circumstances : ( 1 ) when the protein is insoluble in water ...
... medium composed of two or more solvent components present in such ratios that protein - solvent interactions may make important contributions . This is desirable under three circumstances : ( 1 ) when the protein is insoluble in water ...
Page 179
... medium , and is the turbidity of the me- dium . Since , as a practical matter , is often quite small , being of the order of 10- to 10-5 for a typical globular protein in aqueous solution , the light incident on the cell must be intense ...
... medium , and is the turbidity of the me- dium . Since , as a practical matter , is often quite small , being of the order of 10- to 10-5 for a typical globular protein in aqueous solution , the light incident on the cell must be intense ...
Page 231
... medium . Methacrylate , Araldite , and gelatine are among the ma- terials which have been used and detailed recipes are given by Glauert ( 1965 ) . When an embedding medium is to be used , special care must be taken to ensure that this ...
... medium . Methacrylate , Araldite , and gelatine are among the ma- terials which have been used and detailed recipes are given by Glauert ( 1965 ) . When an embedding medium is to be used , special care must be taken to ensure that this ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone