Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 54
... molecular weight as well as the M and M. values of the original solution . E. EFFECTS OF NONIDEALITY w 2 Globular proteins near their isoelectric points and in buffered solutions of high ionic strength have extremely small values for ...
... molecular weight as well as the M and M. values of the original solution . E. EFFECTS OF NONIDEALITY w 2 Globular proteins near their isoelectric points and in buffered solutions of high ionic strength have extremely small values for ...
Page 67
... weight- average molecular weight will be found . In general , if the total weight concentration is c = c + C2 , where c1 and c2 refer to the concentration of monomer and dimer , respectively , at a point where the weight - average molecular ...
... weight- average molecular weight will be found . In general , if the total weight concentration is c = c + C2 , where c1 and c2 refer to the concentration of monomer and dimer , respectively , at a point where the weight - average molecular ...
Page 159
... weight average molecular weight and an average of R , 2 which might appear at first glance to be a z - average . However , since the radius of gyration is related to the molecular weight in a definite manner for any given shape , the ...
... weight average molecular weight and an average of R , 2 which might appear at first glance to be a z - average . However , since the radius of gyration is related to the molecular weight in a definite manner for any given shape , the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone