Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 226
... optical - null instrument ( Robinson , 1951 ) . Over the area of the reference beam atten- uator corresponding to 0.1 to 1.0 transmittance , the absolute ... optical density 0.10 0.05 0.5 Transmittance 1.0 226 R. D. B. FRASER AND E. SUZUKI.
... optical - null instrument ( Robinson , 1951 ) . Over the area of the reference beam atten- uator corresponding to 0.1 to 1.0 transmittance , the absolute ... optical density 0.10 0.05 0.5 Transmittance 1.0 226 R. D. B. FRASER AND E. SUZUKI.
Page 228
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; x is the fraction of the beam obscured by the ...
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; x is the fraction of the beam obscured by the ...
Page 236
... optical density as ordinate and with ordinate and abscissa expanded or contracted to give a convenient presentation . In addition , the spectral slit width and noise level should be chosen carefully and the operating conditions noted on ...
... optical density as ordinate and with ordinate and abscissa expanded or contracted to give a convenient presentation . In addition , the spectral slit width and noise level should be chosen carefully and the operating conditions noted on ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone