Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 24
... pattern is formed in the plane of the photographic plate . The phase plate is rotated so that the phase border does not inter- sect the light path . The conditions for obtaining a clear undistorted interference pattern are more rigorous ...
... pattern is formed in the plane of the photographic plate . The phase plate is rotated so that the phase border does not inter- sect the light path . The conditions for obtaining a clear undistorted interference pattern are more rigorous ...
Page 31
... pattern corresponding to the least blackened points between the dark fringes on the plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are ...
... pattern corresponding to the least blackened points between the dark fringes on the plate . The plate is aligned on the measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are ...
Page 32
... pattern : 1 , inner reference fringes ; 2 , solution - air meniscus ; 3 , outer reference fringes ; the figures mark whole fringes measured from the pure solvent zone near the meniscus . ( b ) Transport pattern ; the figures mark whole ...
... pattern : 1 , inner reference fringes ; 2 , solution - air meniscus ; 3 , outer reference fringes ; the figures mark whole fringes measured from the pure solvent zone near the meniscus . ( b ) Transport pattern ; the figures mark whole ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone