Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 63
... peak is found in the schlieren pattern . If the concentra- tion is increased , the peak on the lower concentration side remains con- stant in area and s value , whereas the other peak increases both in area and s value ( Fig . 22 ) ...
... peak is found in the schlieren pattern . If the concentra- tion is increased , the peak on the lower concentration side remains con- stant in area and s value , whereas the other peak increases both in area and s value ( Fig . 22 ) ...
Page 343
... peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in RNase A identified this peak as His - 48 which is the histidine in a " buried " and ...
... peak 2 and therefore His - 112 to peak 3. The anomalous chemical shift in the acid pH region of peak 4 ( Fig . 33 ) , and its broad line width in RNase A identified this peak as His - 48 which is the histidine in a " buried " and ...
Page 439
... peak in a thermogram . Endothermic reactions are represented by a peak extending below the basline , and exothermic reactions by a peak projecting upward . The peaks on a thermo- gram are frequently identified by the peak tips or the ...
... peak in a thermogram . Endothermic reactions are represented by a peak extending below the basline , and exothermic reactions by a peak projecting upward . The peaks on a thermo- gram are frequently identified by the peak tips or the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone