Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 16
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling ...
... solution over the solvent against which it was dialyzed . c is the total concentration ( gm / ml ) of protein ... protein is sedimented in the absence of added low molec- ular weight electrolyte , because of the strong electrostatic coupling ...
Page 20
... protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window . ( f ) Type III center ...
... protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window . ( f ) Type III center ...
Page 69
... solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity sedimentation experiments are carried out in such a way that a narrow band or zone of protein sediments into ...
... solution is measured ( Fig . 2 ) . In such experiments the protein molecules sediment into a protein solution . Zonal velocity sedimentation experiments are carried out in such a way that a narrow band or zone of protein sediments into ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone