Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 93
... range of 1500 to 75,000 rpm , and the speed of rotation can be set to any desired value within these limits with an accuracy of ± 30 rpm . The speed obtained is indicated by a continuous digital readout which shows a mean speed ...
... range of 1500 to 75,000 rpm , and the speed of rotation can be set to any desired value within these limits with an accuracy of ± 30 rpm . The speed obtained is indicated by a continuous digital readout which shows a mean speed ...
Page 216
... Range , Accuracy , and Resolution For the study of the fundamental bands of proteins , a frequency range of about 250-4000 cm - 1 and accuracies of better than 1⁄2 cm - 1 from 250-2000 cm - 1 and ± 1 cm - 1 between 2000 and 4000 cm - 1 ...
... Range , Accuracy , and Resolution For the study of the fundamental bands of proteins , a frequency range of about 250-4000 cm - 1 and accuracies of better than 1⁄2 cm - 1 from 250-2000 cm - 1 and ± 1 cm - 1 between 2000 and 4000 cm - 1 ...
Page 217
... ranges . Earlier studies of overtone and combination bands in the range 4000- 7000 cm - 1 were conveniently performed by using a silica prism in a single beam infrared spectrometer such as the Perkin - Elmer model 12C , but neither the ...
... ranges . Earlier studies of overtone and combination bands in the range 4000- 7000 cm - 1 were conveniently performed by using a silica prism in a single beam infrared spectrometer such as the Perkin - Elmer model 12C , but neither the ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone