Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 153
... respectively . In this way , in dilute solution , we obtain the familiar light scattering equa- tion ( P. Debye , 1947 ) , shown here in three commonly used forms 1 In Y2 = [ 1 + ( mr ) c . ] = С2 KC 2 R. C2 M2 KC2 1 C2 aμ2 ( e ) 1+ RT ...
... respectively . In this way , in dilute solution , we obtain the familiar light scattering equa- tion ( P. Debye , 1947 ) , shown here in three commonly used forms 1 In Y2 = [ 1 + ( mr ) c . ] = С2 KC 2 R. C2 M2 KC2 1 C2 aμ2 ( e ) 1+ RT ...
Page 251
... respectively ( Darmon and Sutherland , 1947 ) . The frequency of the NH stretching mode is considerably lower and the half - bandwidth considerably greater than is observed in dilute solutions of amides in nonpolar solvents . Studies of ...
... respectively ( Darmon and Sutherland , 1947 ) . The frequency of the NH stretching mode is considerably lower and the half - bandwidth considerably greater than is observed in dilute solutions of amides in nonpolar solvents . Studies of ...
Page 406
... respectively . The full pH dependencies are shown in Fig . 5. This figure shows striking shifts in the proton binding of the histidine residues 12 and 119 , which are implicated in the binding of the inhibitor on other grounds , but no ...
... respectively . The full pH dependencies are shown in Fig . 5. This figure shows striking shifts in the proton binding of the histidine residues 12 and 119 , which are implicated in the binding of the inhibitor on other grounds , but no ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone