Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 92
... rotor hangs on a flexible shaft which makes accurate balancing of the cell and counterbalance less important ( Fig . 32 ) . The maximum centrifugal ... rotor suspended in the rotor chamber of the Model E ultracentrifuge . 92 J. H. COATES.
... rotor hangs on a flexible shaft which makes accurate balancing of the cell and counterbalance less important ( Fig . 32 ) . The maximum centrifugal ... rotor suspended in the rotor chamber of the Model E ultracentrifuge . 92 J. H. COATES.
Page 93
... rotor . Low temperatures ( -5 ° C ) are obtained by means of a refrigeration sys- tem with cooling coils inside the rotor chamber which is normally evac- uated to a pressure of 1 μ of mercury . 2. The M.S.E. Analytical Ultracentrifuge3 ...
... rotor . Low temperatures ( -5 ° C ) are obtained by means of a refrigeration sys- tem with cooling coils inside the rotor chamber which is normally evac- uated to a pressure of 1 μ of mercury . 2. The M.S.E. Analytical Ultracentrifuge3 ...
Page 94
... Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated ...
... Rotor speeds of up to 60,000 rpm are possible , giving a maximum centrifugal force similar to the M.S.E. machine . The rotor speed is electronically controlled and is measured to an accuracy of ± 10 rpm . The rotor can be thermostated ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone