Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 5
... sedimentation velocity experiment a protein solution is subjected to a centrifugal field of about 250,000 g ... sedimentation velocity experiments , it is usual to quote the sedimentation coefficient of a solute ; that is , the ob ...
... sedimentation velocity experiment a protein solution is subjected to a centrifugal field of about 250,000 g ... sedimentation velocity experiments , it is usual to quote the sedimentation coefficient of a solute ; that is , the ob ...
Page 75
... sedimentation , is reduced or disappears on repeating the experiment at a lower speed . For preformed density gradients the speed may be chosen to give the shortest convenient sedimentation time . 2. Measurement of Band Position ...
... sedimentation , is reduced or disappears on repeating the experiment at a lower speed . For preformed density gradients the speed may be chosen to give the shortest convenient sedimentation time . 2. Measurement of Band Position ...
Page 77
... sedimentation coefficient . In this case the rate of sedimentation at the leading and trailing edges of the zone will be less than in the middle of the zone , leading to a steep leading edge and a less steep trailing edge ( Fig . 29 ) ...
... sedimentation coefficient . In this case the rate of sedimentation at the leading and trailing edges of the zone will be less than in the middle of the zone , leading to a steep leading edge and a less steep trailing edge ( Fig . 29 ) ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone