Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 48
... speed from Eq . ( 33 ) . For the high speed method , Yphantis ( 1964 ) recommends that co / ca = 5 so that once again if M is known approximately , the speed may be cal- culated . Following Van Holde ( 1967 ) , graphs of speed against ...
... speed from Eq . ( 33 ) . For the high speed method , Yphantis ( 1964 ) recommends that co / ca = 5 so that once again if M is known approximately , the speed may be cal- culated . Following Van Holde ( 1967 ) , graphs of speed against ...
Page 94
... speeds and centrifugal forces are identical , and the minimum speed is 6000 rpm . The rotor operates in a vacuum at a pressure of 1 μ of mercury and at temperatures up to 150 ° C , controlled to 0.2 ° C . The rotor temperature is ...
... speeds and centrifugal forces are identical , and the minimum speed is 6000 rpm . The rotor operates in a vacuum at a pressure of 1 μ of mercury and at temperatures up to 150 ° C , controlled to 0.2 ° C . The rotor temperature is ...
Page 219
... Speed Control . The speed at which a spectrum may be scanned without introducing errors is limited by electrical damping and mechanical inertia in the photometer and recording units and by the maxi- mum rate of change of transmittance ...
... Speed Control . The speed at which a spectrum may be scanned without introducing errors is limited by electrical damping and mechanical inertia in the photometer and recording units and by the maxi- mum rate of change of transmittance ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone