Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 113
... SPHERES When particles which are much larger than solvent molecules are sus- pended in a liquid flowing through a ... spherical molecules was calculated by Einstein ( 1906 , 1911 ) to be nsp = 2.5 ยข ( 21 ) where is the fraction of the ...
... SPHERES When particles which are much larger than solvent molecules are sus- pended in a liquid flowing through a ... spherical molecules was calculated by Einstein ( 1906 , 1911 ) to be nsp = 2.5 ยข ( 21 ) where is the fraction of the ...
Page 117
... spherical beads ( Kirkwood , 1949 ; Kirkwood and Riseman , 1950 ; Kirk- wood and Auer , 1951 ) . Treatments ( 1 ) and ( 2 ) give similar results if the axial ratio is high . Under these conditions the prolate ellipsoid is a rea- sonable ...
... spherical beads ( Kirkwood , 1949 ; Kirkwood and Riseman , 1950 ; Kirk- wood and Auer , 1951 ) . Treatments ( 1 ) and ( 2 ) give similar results if the axial ratio is high . Under these conditions the prolate ellipsoid is a rea- sonable ...
Page 134
... spherical bead model ( using experimental values of [ n ] and so in each case ) and this is compared with the particle ... sphere accord- ing to Eq . ( 28 ) , and they increase markedly on denaturation . However , the intrinsic viscosity ...
... spherical bead model ( using experimental values of [ n ] and so in each case ) and this is compared with the particle ... sphere accord- ing to Eq . ( 28 ) , and they increase markedly on denaturation . However , the intrinsic viscosity ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone