Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 72
... wavelength are most readily studied . Re- fractometric optics are not well suited to zonal velocity sedimentation since they do not differentiate between the protein solute and the low molecular weight substance used for the stabilizing ...
... wavelength are most readily studied . Re- fractometric optics are not well suited to zonal velocity sedimentation since they do not differentiate between the protein solute and the low molecular weight substance used for the stabilizing ...
Page 93
... wavelength . The transmitted intensity is recorded by means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can ...
... wavelength . The transmitted intensity is recorded by means of a photomultiplier which mechanically scans the image of the ultracen- trifuge cell . The logarithm of the photocurrent is plotted by means of a pen recorder . The system can ...
Page 179
... wavelength . Hence , the shorter blue and green wavelengths , in which the mercury arc is particularly rich , are the usual choices for maximizing the amount of scattered light . These wavelengths are dictated also by other factors ...
... wavelength . Hence , the shorter blue and green wavelengths , in which the mercury arc is particularly rich , are the usual choices for maximizing the amount of scattered light . These wavelengths are dictated also by other factors ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone