Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 17
Page 71
... zone ; consequently the resolution of protein zones is not as good as that of nucleic acid zones . Proteins also suffer from the disadvantage that , compared with nucleic acids , higher concentrations are needed for their ...
... zone ; consequently the resolution of protein zones is not as good as that of nucleic acid zones . Proteins also suffer from the disadvantage that , compared with nucleic acids , higher concentrations are needed for their ...
Page 76
... zone is equal to cm / √2 . Cm is the maximum concentration in the zone . In practice , for narrow zones there is little error incurred if the sedi- mentation rate is taken as the rate of movement of the maximum , and s is determined ...
... zone is equal to cm / √2 . Cm is the maximum concentration in the zone . In practice , for narrow zones there is little error incurred if the sedi- mentation rate is taken as the rate of movement of the maximum , and s is determined ...
Page 77
... zone will be less than in the middle of the zone , leading to a steep leading edge and a less steep trailing edge ( Fig . 29 ) . 4. Conversion of s Values to Standard Conditions Since zone sedimentation is of necessity carried out in ...
... zone will be less than in the middle of the zone , leading to a steep leading edge and a less steep trailing edge ( Fig . 29 ) . 4. Conversion of s Values to Standard Conditions Since zone sedimentation is of necessity carried out in ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone