Molecular Cloning: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction

agarose gel aliquots amplified DNA annealing antibody Appendix bacteriophage T4 Biol BioTechniques catalyzed cDNA clones cDNA libraries cDNA synthesis cells centrifuge Chapter concentration containing cycle denaturation detection digestion DNA fragments DNA ligase DNA sequencing DNA templates dNTPs double-stranded DNA efficiency encoding ethanol exonuclease expression filters first-strand cDNA gel electrophoresis gene genomic DNA H₂O hybridization Incubate information panel Klenow fragment labeled length ligation mammalian method microfuge tube minutes molecules mRNA mutagenesis mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products plaques plasmid plasmid DNA plates polyacrylamide gel polymerase chain reaction preparation probes protein Protocol purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase H room temperature screening sequencing reactions single-stranded DNA Step strand T4 DNA target DNA template DNA termini thermal cycler thermostable DNA polymerase tion transcription vector vitro µg/ml