Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 92
... angle meridional streaks or reflections near the center of the diffraction pat- tern at diffraction angles less than about 4 ° . The layer lines on which these streaks occur are close together because of the large repeat distance ...
... angle meridional streaks or reflections near the center of the diffraction pat- tern at diffraction angles less than about 4 ° . The layer lines on which these streaks occur are close together because of the large repeat distance ...
Page 182
... angle ẞ between the directions of the two oscillators and , according to the theory of Jablonski ( 1935 ) , is given ... angle during the interval between excitation and emission . The average value of this angle will depend upon the ...
... angle ẞ between the directions of the two oscillators and , according to the theory of Jablonski ( 1935 ) , is given ... angle during the interval between excitation and emission . The average value of this angle will depend upon the ...
Page 336
... angle between the symmetry axis and the field direction rotational diffusion constant wavelength of light in vacuo apparent permanent dipole moment relaxation time orientation factor , ( ( 3 cos2 0 - 1 ) / 2 ) av . angle between the ...
... angle between the symmetry axis and the field direction rotational diffusion constant wavelength of light in vacuo apparent permanent dipole moment relaxation time orientation factor , ( ( 3 cos2 0 - 1 ) / 2 ) av . angle between the ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone