Physical Principles and Techniques of Protein Chemistry, Part 1

absorbance absorption acid appears applied atoms axis binding birefringence boundary buffer calculated cell charge Chem chromophores complex components concentration constant containing corrected corresponding curve dependence determined dielectric difference diffusion dipole direction distribution effect electric field electron electrophoresis elution emission energy equation equilibrium et al example excitation experimental experiments factor flow fluorescence fraction frequency function gel filtration given groups important increase indicate intensity interactions interpretation ionic strength ions light limited macromolecules measured method migration mixture mobility molecular molecules moving-boundary observed obtained occurs optical orientation particles patterns peaks perturbation phase plot polarization position possible present procedure produced protein ratio reaction reference relative relaxation respectively rotational separation serum albumin shown single solution solvent specific spectra spectrum structure studies technique theoretical theory tion unit usually values volume wavelength weight yield zone