Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 338
... birefringence of colloidal solutions in pulsed fields was initiated independently by Kaye and Devaney ( 1947 ) ... birefringence produced changes with time as shown in Fig . 1. If the pulse duration is adjusted so that the steady - state ...
... birefringence of colloidal solutions in pulsed fields was initiated independently by Kaye and Devaney ( 1947 ) ... birefringence produced changes with time as shown in Fig . 1. If the pulse duration is adjusted so that the steady - state ...
Page 358
... birefringent in an electric field . Subsequently , Lauffer ( 1939 ) investigated the electric birefringence in an alternating field of 60 cycles / sec . Since the electric birefringence , as well as the flow birefringence , of dilute ...
... birefringent in an electric field . Subsequently , Lauffer ( 1939 ) investigated the electric birefringence in an alternating field of 60 cycles / sec . Since the electric birefringence , as well as the flow birefringence , of dilute ...
Page 362
... birefringence of soluble collagen in dilute acetic acid . Pronounced saturation of the bire- fringence was observed , permitting calculation of the optical anisotropy factor . From the field strength dependence of the birefringence ...
... birefringence of soluble collagen in dilute acetic acid . Pronounced saturation of the bire- fringence was observed , permitting calculation of the optical anisotropy factor . From the field strength dependence of the birefringence ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone