Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 109
... chromophore and shift its absorption spectrum along the wavelength scale . The change is de- termined both by the nature of the electronic transition and by the relative contributions of chromophore - solvent interactions to the ...
... chromophore and shift its absorption spectrum along the wavelength scale . The change is de- termined both by the nature of the electronic transition and by the relative contributions of chromophore - solvent interactions to the ...
Page 131
... chromophore is carried out , not only will the correct estimate of exposure of the chromophores in the protein . be obtained , but the preferential solvation of the chromophore will go undetected . If preferential solvation by the ...
... chromophore is carried out , not only will the correct estimate of exposure of the chromophores in the protein . be obtained , but the preferential solvation of the chromophore will go undetected . If preferential solvation by the ...
Page 146
... chromophore near an active site , or near a region which undergoes a conformational change , or on a surface at which association takes place , then an artificial chromophore may be inserted by chemical modification of the protein ...
... chromophore near an active site , or near a region which undergoes a conformational change , or on a surface at which association takes place , then an artificial chromophore may be inserted by chemical modification of the protein ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone