Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 45
... corrections of this kind does not seem to have been generally recognized . Particle diameters quoted in the literature must be suitably adjusted if no correction is noted by the author . Since the correction for the accumulation of ...
... corrections of this kind does not seem to have been generally recognized . Particle diameters quoted in the literature must be suitably adjusted if no correction is noted by the author . Since the correction for the accumulation of ...
Page 198
... correction at- tachment for the Aminco - Bowman instrument . Excitation spectra are corrected as follows : a part of the light leaving the first monochromator is deflected with a beam - splitter onto a thermopile . Energy changes are ...
... correction at- tachment for the Aminco - Bowman instrument . Excitation spectra are corrected as follows : a part of the light leaving the first monochromator is deflected with a beam - splitter onto a thermopile . Energy changes are ...
Page 199
... corrected with a dozen or so points . A good general discussion of the necessary corrections is that of Parker and Rees ... Correction of the excitation spectrum . A calibration curve to show the relative photon output of the xenon lamp ...
... corrected with a dozen or so points . A good general discussion of the necessary corrections is that of Parker and Rees ... Correction of the excitation spectrum . A calibration curve to show the relative photon output of the xenon lamp ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone