Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 286
... Section AB 3335 34 5 4 3 2 22 44 47 48 16 49 17 50 61 51 62 56 58 2017 36 37 38 39 43 42 41 40 33 32 38 59 60 13 14 15 19 21 22 23 30 31 FIG . 15a . Cross section of the flow - temperature - jump unit . 5 , high - voltage elec- trode ...
... Section AB 3335 34 5 4 3 2 22 44 47 48 16 49 17 50 61 51 62 56 58 2017 36 37 38 39 43 42 41 40 33 32 38 59 60 13 14 15 19 21 22 23 30 31 FIG . 15a . Cross section of the flow - temperature - jump unit . 5 , high - voltage elec- trode ...
Page 382
Sydney J. Leach. U - tube , rectangular in cross section , with end walls of high optical quality to permit observation of boundaries by changes in the refractive index along the length of the cell . The cell is composed of three sections ...
Sydney J. Leach. U - tube , rectangular in cross section , with end walls of high optical quality to permit observation of boundaries by changes in the refractive index along the length of the cell . The cell is composed of three sections ...
Page 492
... cross section subjected to a uniform field . Substitution of sedimentation coefficients ( s ) for elution volumes in Eq . ( 35 ) yields a moving - boundary equation which applies only imperfectly to velocity sedimentation , where a ...
... cross section subjected to a uniform field . Substitution of sedimentation coefficients ( s ) for elution volumes in Eq . ( 35 ) yields a moving - boundary equation which applies only imperfectly to velocity sedimentation , where a ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Operational Requirements for HighResolution Electron | 15 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis binding Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone