Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 328
... defined by the following equation : Δες Δεν = ∞ n2G ( n ) dn 1+ w2B2no ∞0 n2G ( n ) dn ( 86 ) where Bn3 represents the relaxation time , G ( n ) is a distribution function , and Aet is the total dielectric increment . Defining a new ...
... defined by the following equation : Δες Δεν = ∞ n2G ( n ) dn 1+ w2B2no ∞0 n2G ( n ) dn ( 86 ) where Bn3 represents the relaxation time , G ( n ) is a distribution function , and Aet is the total dielectric increment . Defining a new ...
Page 513
... definition of , 309 of polypeptides , 331-332 of proteins , 311 Dielectric loss definition of , 303 in dielectric perturbation , 253-255 measurement of , 307-310 Dielectric relaxation , 291-333 activation energy parameters for , 307 of ...
... definition of , 309 of polypeptides , 331-332 of proteins , 311 Dielectric loss definition of , 303 in dielectric perturbation , 253-255 measurement of , 307-310 Dielectric relaxation , 291-333 activation energy parameters for , 307 of ...
Page 514
... definition of , 336 detection system for , 354 of ellipsoids of revolution , 344-346 of fibrous proteins , 361–362 of globular proteins , 362-363 of macromolecules in reversing fields , 348 measurement of , 350-357 optical dispersion of ...
... definition of , 336 detection system for , 354 of ellipsoids of revolution , 344-346 of fibrous proteins , 361–362 of globular proteins , 362-363 of macromolecules in reversing fields , 348 measurement of , 350-357 optical dispersion of ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone