Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 151
... determined mainly from the absorption near 295 mμ , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mu . ) The method of Edelhoch is better suited for the determination of tryptophan Мтер = 10 ...
... determined mainly from the absorption near 295 mμ , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mu . ) The method of Edelhoch is better suited for the determination of tryptophan Мтер = 10 ...
Page 163
... determined . If the perturbant produces an increase in absorption , the contents of the two compartments of the ... determined by the thickness of a gasket of inert material are also available commercially . The actual path length of ...
... determined . If the perturbant produces an increase in absorption , the contents of the two compartments of the ... determined by the thickness of a gasket of inert material are also available commercially . The actual path length of ...
Page 354
... determined by dif- ference in the Helipot readings . The time constant of the detecting system is determined mostly by the distributed capacitance and the anode resistance in the photo- multiplier circuit . It is essential to reduce the ...
... determined by dif- ference in the Helipot readings . The time constant of the detecting system is determined mostly by the distributed capacitance and the anode resistance in the photo- multiplier circuit . It is essential to reduce the ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Operational Requirements for HighResolution Electron | 15 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis binding Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone