Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 380
... diffusion by an external force is called forced diffusion . The fundamental differential equation of forced diffusion is obtained by modification of Fick's second law of diffusion to take into account the additional flow produced by the ...
... diffusion by an external force is called forced diffusion . The fundamental differential equation of forced diffusion is obtained by modification of Fick's second law of diffusion to take into account the additional flow produced by the ...
Page 441
... Diffusion in the Case of Electrophoresis The differential equation of forced diffusion , sometimes referred to as the conservation equation , is simply a statement of the conservation of mass during transport caused by an external force ...
... Diffusion in the Case of Electrophoresis The differential equation of forced diffusion , sometimes referred to as the conservation equation , is simply a statement of the conservation of mass during transport caused by an external force ...
Page 443
... diffusion " are seen with greatest clarity upon transformation of Eq . ( A10 ) to the moving coordinate system , y = x — μEt , ac at = D a2C Əy2 ( A11 ) This transformed equation is Fick's second law for diffusion in the moving ...
... diffusion " are seen with greatest clarity upon transformation of Eq . ( A10 ) to the moving coordinate system , y = x — μEt , ac at = D a2C Əy2 ( A11 ) This transformed equation is Fick's second law for diffusion in the moving ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone