Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 111
... dipole tion tion nent Net structure Case phore vent moment dipole effects dipole shift is I N N None Red None None Red Retained II N Р None Red None None Red Blurred IIIa Р N Decrease Red None Blue C Retained IIIb Р N Increase Red None ...
... dipole tion tion nent Net structure Case phore vent moment dipole effects dipole shift is I N N None Red None None Red Retained II N Р None Red None None Red Blurred IIIa Р N Decrease Red None Blue C Retained IIIb Р N Increase Red None ...
Page 215
... dipole moment after it has absorbed a photon to reach the excited state . After excitation , there is a reorientation of solvent dipoles in the vicinity of the solute molecule . The magnitude of the dipole reorientation , which expends ...
... dipole moment after it has absorbed a photon to reach the excited state . After excitation , there is a reorientation of solvent dipoles in the vicinity of the solute molecule . The magnitude of the dipole reorientation , which expends ...
Page 295
... DIPOLE MOMENT If a negative charge —q ; and a positive charge + q ; exist at a distance r ; from each other and if they satisfy the condition Σ qi 0 = then the dipole moment μ is defined by the following equation μ = qiri ( 1 ) ( 2 ) If ...
... DIPOLE MOMENT If a negative charge —q ; and a positive charge + q ; exist at a distance r ; from each other and if they satisfy the condition Σ qi 0 = then the dipole moment μ is defined by the following equation μ = qiri ( 1 ) ( 2 ) If ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone