Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 25
... distance of evaporation source from specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of ...
... distance of evaporation source from specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of ...
Page 222
... distance of 17 Å was determined by Weber ( 1960a ) which is in approximate accord with that calculated from Förster's theory . Energy transfer would therefore be expected to occur in small proteins containing several tyrosyl residues ...
... distance of 17 Å was determined by Weber ( 1960a ) which is in approximate accord with that calculated from Förster's theory . Energy transfer would therefore be expected to occur in small proteins containing several tyrosyl residues ...
Page 309
... distance 1 and distance 2 ( d1 > d2 ) and R1 and R2 are the resistances ( ohms ) at these electrode distances . To obtain satisfactory results , the distance d1 must . be considerably larger than de . Since measurements at large electrode ...
... distance 1 and distance 2 ( d1 > d2 ) and R1 and R2 are the resistances ( ohms ) at these electrode distances . To obtain satisfactory results , the distance d1 must . be considerably larger than de . Since measurements at large electrode ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone