Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 302
... Electric field Electric polarization P ( t ) E ( t ) I POD = E∞ + 1 + ίωτ t FIG . 1. Schematic representation of an electric field and the polarization response . The upper curve represents the change in electric field intensity when ...
... Electric field Electric polarization P ( t ) E ( t ) I POD = E∞ + 1 + ίωτ t FIG . 1. Schematic representation of an electric field and the polarization response . The upper curve represents the change in electric field intensity when ...
Page 338
... electric field . The orientation may be caused by a permanent dipole moment of the molecule or its electrical ... electric birefringence of colloidal solutions in pulsed fields was initiated independently by Kaye and Devaney ( 1947 ) ...
... electric field . The orientation may be caused by a permanent dipole moment of the molecule or its electrical ... electric birefringence of colloidal solutions in pulsed fields was initiated independently by Kaye and Devaney ( 1947 ) ...
Page 514
... electric birefringence for , 339 ff . of excited states , 215 of polypeptides , 326-332 , 363-365 of proteins calculation of , 311-312 fluctuation of , 316-320 interpretation of , 312 pH effects on , 316-319 size effect on , 321-322 ...
... electric birefringence for , 339 ff . of excited states , 215 of polypeptides , 326-332 , 363-365 of proteins calculation of , 311-312 fluctuation of , 316-320 interpretation of , 312 pH effects on , 316-319 size effect on , 321-322 ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone