Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 174
... energy of the molecule . If all electrons in the mole- cule have paired spins it is said to be in the singlet state . This is the case for most organic molecules in the ground state . If one set of electrons has unpaired spins the ...
... energy of the molecule . If all electrons in the mole- cule have paired spins it is said to be in the singlet state . This is the case for most organic molecules in the ground state . If one set of electrons has unpaired spins the ...
Page 178
... energy may be emitted with the fluorescence spectrum characteristic of the second molecule . The process has been variously termed radiationless transfer , radiationless energy exchange , or electronic energy transfer ( Förster , 1951 ) ...
... energy may be emitted with the fluorescence spectrum characteristic of the second molecule . The process has been variously termed radiationless transfer , radiationless energy exchange , or electronic energy transfer ( Förster , 1951 ) ...
Page 195
... energy instead of relative quanta . The energy of a quantum , multi- plied by the total number of quanta , gives the total energy . Spectra expressed in terms of energy tend to give more emphasis on the ultra- violet , since a photon of ...
... energy instead of relative quanta . The energy of a quantum , multi- plied by the total number of quanta , gives the total energy . Spectra expressed in terms of energy tend to give more emphasis on the ultra- violet , since a photon of ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone