Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 249
... equilibrium position characterized by a higher ( usually by 5 ° -10 ° C ) temperature . Similarly , the ... equilibrium . Since the equilibrium constant for this reaction is assumed to be temperature dependent , the equilibrium ...
... equilibrium position characterized by a higher ( usually by 5 ° -10 ° C ) temperature . Similarly , the ... equilibrium . Since the equilibrium constant for this reaction is assumed to be temperature dependent , the equilibrium ...
Page 482
... equilibrium . However , the gel filtration data on aryl sulfatase A ( Fig . 7 ) serve to emphasize that the difference may only reflect the magnitude of the association constant in relation to the pro- tein concentration used for ...
... equilibrium . However , the gel filtration data on aryl sulfatase A ( Fig . 7 ) serve to emphasize that the difference may only reflect the magnitude of the association constant in relation to the pro- tein concentration used for ...
Page 492
... equilibrium concentrations of B directly for this combination of elution volumes ( VC = VA < VB ) . Thus , although ... equilibrium concentra- tion of B is obtained directly from the trailing profile for systems con- forming with this ...
... equilibrium concentrations of B directly for this combination of elution volumes ( VC = VA < VB ) . Thus , although ... equilibrium concentra- tion of B is obtained directly from the trailing profile for systems con- forming with this ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone