Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 98
... et al . ( 1960 ) , Marvin et al . ( 1961 ) , and Bradbury et al . ( 1965 ) . The methods outlined in Section V may be used to calculate the pattern of a crystalline fiber , while those in Section VIII , D may be used to calculate the ...
... et al . ( 1960 ) , Marvin et al . ( 1961 ) , and Bradbury et al . ( 1965 ) . The methods outlined in Section V may be used to calculate the pattern of a crystalline fiber , while those in Section VIII , D may be used to calculate the ...
Page 229
... et al . , 1968 ) . Ionization of the normal tyrosyl resi- dues in ribonuclease quenches the blocked tyrosyl residues ( Cowgill , 1965a ) . Quenching of tryptophyl group fluorescence by base occurs above pH 12 and is to be differentiated ...
... et al . , 1968 ) . Ionization of the normal tyrosyl resi- dues in ribonuclease quenches the blocked tyrosyl residues ( Cowgill , 1965a ) . Quenching of tryptophyl group fluorescence by base occurs above pH 12 and is to be differentiated ...
Page 398
... et al . , 1948 ; Anderson and Alberty , 1948 ; Baldwin et al . , 1951 ) R. A. Brown and Cann ( 1950 ) solved Eq . ( 18 ) for non - Gaussian mobility distribu- tions and showed that the mobility distribution can be expressed in terms of ...
... et al . , 1948 ; Anderson and Alberty , 1948 ; Baldwin et al . , 1951 ) R. A. Brown and Cann ( 1950 ) solved Eq . ( 18 ) for non - Gaussian mobility distribu- tions and showed that the mobility distribution can be expressed in terms of ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone