Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 174
... fluorescence . Excitation ordinarily occurs from the ground state , which is the nor- mal state of lowest energy of the molecule . If all electrons in the mole- cule have paired spins it is said to be in the singlet state . This is the ...
... fluorescence . Excitation ordinarily occurs from the ground state , which is the nor- mal state of lowest energy of the molecule . If all electrons in the mole- cule have paired spins it is said to be in the singlet state . This is the ...
Page 229
... fluorescence was completely quenched when only a single tyrosyl group was ionized ( Edelhoch et al . , 1968 ) . Ionization of the normal tyrosyl resi- dues in ribonuclease quenches the blocked tyrosyl residues ( Cowgill , 1965a ) ...
... fluorescence was completely quenched when only a single tyrosyl group was ionized ( Edelhoch et al . , 1968 ) . Ionization of the normal tyrosyl resi- dues in ribonuclease quenches the blocked tyrosyl residues ( Cowgill , 1965a ) ...
Page 230
... fluorescence , or polarization of fluorescence data ( Edelhoch and Steiner , 1963 ) . It is clear that since the rate constants obtained from several independent methods are identical , a single rate - determining event is responsible ...
... fluorescence , or polarization of fluorescence data ( Edelhoch and Steiner , 1963 ) . It is clear that since the rate constants obtained from several independent methods are identical , a single rate - determining event is responsible ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone