Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 120
... histidine methyl ester , pH 6.6 vs. pH 4.1 ( left ordinate ) ; ...... ribonuclease , pH 6.5 vs. pH 5.0 , in water , uncorrected for superimposed phenolic perturbation ( right ordinate ) ( Donovan , 1965 ) . ( Courtesy American Chemical ...
... histidine methyl ester , pH 6.6 vs. pH 4.1 ( left ordinate ) ; ...... ribonuclease , pH 6.5 vs. pH 5.0 , in water , uncorrected for superimposed phenolic perturbation ( right ordinate ) ( Donovan , 1965 ) . ( Courtesy American Chemical ...
Page 126
... histidine . 2.0 6 Molar absorption differences ( A x 10 ̃3 ) 1.5 1.0- 150 2.4 3.5 8.1 9.0 -0.5 9.5 10.5 11.0 220 240 260 280 Wavelength ( mμ ) 300 320 2 ( e x 10-4 ) FIG . 10. Difference spectra of tryptophan at 22 ° . Samples at the pH ...
... histidine . 2.0 6 Molar absorption differences ( A x 10 ̃3 ) 1.5 1.0- 150 2.4 3.5 8.1 9.0 -0.5 9.5 10.5 11.0 220 240 260 280 Wavelength ( mμ ) 300 320 2 ( e x 10-4 ) FIG . 10. Difference spectra of tryptophan at 22 ° . Samples at the pH ...
Page 128
... Histidine Imidazole Amino 228 825 0.145 Histidine Imidazole Imino 226 -285 0.050 Phenylalanined Benzene Carboxyl 259 4 0.022 3 Phenylalanined Benzene Amino 260 14 0.07 O - Methyltyrosine Phenol Carboxyl 284 55 0.039 3.2 O ...
... Histidine Imidazole Amino 228 825 0.145 Histidine Imidazole Imino 226 -285 0.050 Phenylalanined Benzene Carboxyl 259 4 0.022 3 Phenylalanined Benzene Amino 260 14 0.07 O - Methyltyrosine Phenol Carboxyl 284 55 0.039 3.2 O ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone