Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 86
Page 123
... increase of 15 % in intrinsic viscosity suggested that a minor conformational change occurred . The fluorescence yield of the phenolic groups of ovomucoid increased several hundred percent over the same pH range . It appears that the ...
... increase of 15 % in intrinsic viscosity suggested that a minor conformational change occurred . The fluorescence yield of the phenolic groups of ovomucoid increased several hundred percent over the same pH range . It appears that the ...
Page 227
... increase in tyrosyl emission occurs in the acid transition of ribonuclease which runs parallel with optical rotation and difference absorption spectra and presumably results from the nor- malization of the three blocked tyrosyl groups ...
... increase in tyrosyl emission occurs in the acid transition of ribonuclease which runs parallel with optical rotation and difference absorption spectra and presumably results from the nor- malization of the three blocked tyrosyl groups ...
Page 232
... increase with any deviation from a compact , rigid sphere whereas the relaxation time ratio will increase only if the molecule becomes asymmetric while remaining completely rigid . The acquisition of rotational degrees of freedom will ...
... increase with any deviation from a compact , rigid sphere whereas the relaxation time ratio will increase only if the molecule becomes asymmetric while remaining completely rigid . The acquisition of rotational degrees of freedom will ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
64 other sections not shown
Other editions - View all
Common terms and phrases
absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone