Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 50
... length of fibrinogen molecules as a function of pH seems to indicate that contraction of the molecule to a length of 230 Å is in fact possible ( H. S. Slayter and Hall , 1962 ) . Measurements from electron micrographs , of specimens ...
... length of fibrinogen molecules as a function of pH seems to indicate that contraction of the molecule to a length of 230 Å is in fact possible ( H. S. Slayter and Hall , 1962 ) . Measurements from electron micrographs , of specimens ...
Page 163
... length cell and a more concentrated solution in a short - path - length cell ( Section VI , G ) , or merely to use short - path - length cells because other experimental condi- tions ( e.g. , high concentration ) require the use of ...
... length cell and a more concentrated solution in a short - path - length cell ( Section VI , G ) , or merely to use short - path - length cells because other experimental condi- tions ( e.g. , high concentration ) require the use of ...
Page 365
... length and the length distribution of the macromolecule were calculated by a parameter method developed for analyzing the decay curve . The increase of water content caused the decrease of the mean length and broadened the length ...
... length and the length distribution of the macromolecule were calculated by a parameter method developed for analyzing the decay curve . The increase of water content caused the decrease of the mean length and broadened the length ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone