Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 427
... migration of zones are often very marked indeed . For example , during paper electrophoresis of serum at pH 8.6 , the y - globulin zone generally migrates toward the cathode despite the fact that the y - globulin molecule is negatively ...
... migration of zones are often very marked indeed . For example , during paper electrophoresis of serum at pH 8.6 , the y - globulin zone generally migrates toward the cathode despite the fact that the y - globulin molecule is negatively ...
Page 431
... migration rate of the protein . above the boundary equal that of the intrinsically faster chloride ion beneath the boundary . ) Upon entering the separating gel , which is at a higher pH than the tris - glycine buffer bathing the two ...
... migration rate of the protein . above the boundary equal that of the intrinsically faster chloride ion beneath the boundary . ) Upon entering the separating gel , which is at a higher pH than the tris - glycine buffer bathing the two ...
Page 462
... migration and the diffusion coefficient . Gel filtration provides only one experimental parameter , the partition coefficient , the use of which to define both migration and boundary spreading obviously necessitates the adoption of ...
... migration and the diffusion coefficient . Gel filtration provides only one experimental parameter , the partition coefficient , the use of which to define both migration and boundary spreading obviously necessitates the adoption of ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone