Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 373
... mobility . The dimensions of mobility are cm2 sec - 1 V - 1 , and its sign is the same as that of the net electrical charge on the particle . Although in a given medium the mobility is a characteristic property of the particle , it ...
... mobility . The dimensions of mobility are cm2 sec - 1 V - 1 , and its sign is the same as that of the net electrical charge on the particle . Although in a given medium the mobility is a characteristic property of the particle , it ...
Page 381
... mobility . By means of Eq . ( 12 ) it is a simple matter to show that the area under the gradient curve is proportional to the concentration of the macromolecule and equal to Co and that the electrophoretic mobility of the macromolecule ...
... mobility . By means of Eq . ( 12 ) it is a simple matter to show that the area under the gradient curve is proportional to the concentration of the macromolecule and equal to Co and that the electrophoretic mobility of the macromolecule ...
Page 398
... mobility distribu- tions and showed that the mobility distribution can be expressed in terms of the moments of the gradient curve x taken about the centroidal axis g ( μ ) = [ 1 / ẞ ( 2π ) / 2 ] ( exp -μ2 / 2ẞ2 ) { 1+ - B2 = ( σ2 — 2DtD ) ...
... mobility distribu- tions and showed that the mobility distribution can be expressed in terms of the moments of the gradient curve x taken about the centroidal axis g ( μ ) = [ 1 / ẞ ( 2π ) / 2 ] ( exp -μ2 / 2ẞ2 ) { 1+ - B2 = ( σ2 — 2DtD ) ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone