Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 38
... observation of individual freeze- dried macromolecules . These small particles simply disappear during sublimation . Westerberg ( 1961 ) has , however , observed freeze - dried strands of DNA , and it is possible that in the future ...
... observation of individual freeze- dried macromolecules . These small particles simply disappear during sublimation . Westerberg ( 1961 ) has , however , observed freeze - dried strands of DNA , and it is possible that in the future ...
Page 50
... observed after its activation by thrombin and subsequent polymerization to form fibrin . An apparent difficulty is raised by the observation of fibrinogen molecules as long as 475 Å be- cause the periodicity of fibrin is only 230 A. As ...
... observed after its activation by thrombin and subsequent polymerization to form fibrin . An apparent difficulty is raised by the observation of fibrinogen molecules as long as 475 Å be- cause the periodicity of fibrin is only 230 A. As ...
Page 51
... observed in the pH range from 4.0 to 3.6 . Over the same pH range , a decrease of sedimentation constant and diffusion co- efficient , and an increase in intrinsic viscosity are observed . At neutral pH , however , the bovine serum ...
... observed in the pH range from 4.0 to 3.6 . Over the same pH range , a decrease of sedimentation constant and diffusion co- efficient , and an increase in intrinsic viscosity are observed . At neutral pH , however , the bovine serum ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone