Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 9
... optical paths ( refractive index X distance ) before reaching the image . If variations in optical path occur , variations in the in- tensity of image points are produced by phase interference between co- incident rays which differ in ...
... optical paths ( refractive index X distance ) before reaching the image . If variations in optical path occur , variations in the in- tensity of image points are produced by phase interference between co- incident rays which differ in ...
Page 118
... optical density of a sample solution of total chromophore concen- tration c , measured in a 1 - cm cell , is given by the sum of the optical densities of the ionized and un - ionized forms . In addition to being a function of ...
... optical density of a sample solution of total chromophore concen- tration c , measured in a 1 - cm cell , is given by the sum of the optical densities of the ionized and un - ionized forms . In addition to being a function of ...
Page 336
... optical anisotropy factor light intensity Boltzmann constant Kerr constant , An / nE2 optical path length torque refractive index principal refractive indices parallel and perpendicular to the electric field , respectively birefringence ...
... optical anisotropy factor light intensity Boltzmann constant Kerr constant , An / nE2 optical path length torque refractive index principal refractive indices parallel and perpendicular to the electric field , respectively birefringence ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone