Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 43
Sydney J. Leach. 2. Measurement of Shadow - Cast Particles In preparations contrasted by the shadow - casting method , it is pos- sible to determine the heights of the shadowed particles from the lengths of the shadows cast if the ...
Sydney J. Leach. 2. Measurement of Shadow - Cast Particles In preparations contrasted by the shadow - casting method , it is pos- sible to determine the heights of the shadowed particles from the lengths of the shadows cast if the ...
Page 44
... particles . The accumulation of metal is par- ticularly obvious when groups of particles are in contact before shadow- ing , since the individual members of the group may then seem obviously smaller than isolated particles . The particle ...
... particles . The accumulation of metal is par- ticularly obvious when groups of particles are in contact before shadow- ing , since the individual members of the group may then seem obviously smaller than isolated particles . The particle ...
Page 47
... particles are unusually uniform , significant vari- ations do occur , in contrast to the highly constant spacings between rulings of a diffraction grating or atoms of a crystal . Polystyrene spheres in fact serve two quite different ...
... particles are unusually uniform , significant vari- ations do occur , in contrast to the highly constant spacings between rulings of a diffraction grating or atoms of a crystal . Polystyrene spheres in fact serve two quite different ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone