Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 313
... plot are shown in Fig . 6. Obvi- ously , the dielectric dispersion of myoglobin fits the Debye theory within experimental error . The Cole - Cole plot ( Cole and Cole , 1941 ) is Dielectric constant 88 86 ε " 84 8 88 86 82 80 888 78 100 ...
... plot are shown in Fig . 6. Obvi- ously , the dielectric dispersion of myoglobin fits the Debye theory within experimental error . The Cole - Cole plot ( Cole and Cole , 1941 ) is Dielectric constant 88 86 ε " 84 8 88 86 82 80 888 78 100 ...
Page 465
... plot ( e.g. , Tracey , 1964 ) is obviously to be preferred , but manual differentiation has usually been employed ... plot with that obtained by automatic derivative recording for a - chymotrypsin in 0.01 M phosphate , pH 7.9 ( Tracey ...
... plot ( e.g. , Tracey , 1964 ) is obviously to be preferred , but manual differentiation has usually been employed ... plot with that obtained by automatic derivative recording for a - chymotrypsin in 0.01 M phosphate , pH 7.9 ( Tracey ...
Page 486
... plot or obvious nonconformity of the trailing elution profile with the expected shape ( Bethune and Grillo , 1967 ) , the following procedure may be used to evaluate successive association constants ( Steiner , 1952 ) . In a protein ...
... plot or obvious nonconformity of the trailing elution profile with the expected shape ( Bethune and Grillo , 1967 ) , the following procedure may be used to evaluate successive association constants ( Steiner , 1952 ) . In a protein ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Operational Requirements for HighResolution Electron | 15 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis binding Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone