Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 50
... possible mechanisms , neither of which is unequivocally established as correct at present , could account for these data . As shown in Fig . 12b , the intermediate polymers might be double chains in which the arrangement of the ...
... possible mechanisms , neither of which is unequivocally established as correct at present , could account for these data . As shown in Fig . 12b , the intermediate polymers might be double chains in which the arrangement of the ...
Page 179
... possible radiative deactivation of the donor corre- sponds precisely to that for a possible absorption transition by an acceptor molecule in the vicinity , then a quantum mechanical argument shows that there is a finite probability of a ...
... possible radiative deactivation of the donor corre- sponds precisely to that for a possible absorption transition by an acceptor molecule in the vicinity , then a quantum mechanical argument shows that there is a finite probability of a ...
Page 247
... possible reaction schemes . A great deal of attention must therefore be paid to the elimination of those possible pathways which in fact do not apply . It is also clear that the successful elucidation of the mechanism of chemical ...
... possible reaction schemes . A great deal of attention must therefore be paid to the elimination of those possible pathways which in fact do not apply . It is also clear that the successful elucidation of the mechanism of chemical ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone