Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 24
... procedure is once worked out for use with a particular vacuum evaporator , the method is not only extremely simple , but also remarkably reliable in routine use . Important considerations in establishing a standard procedure include ...
... procedure is once worked out for use with a particular vacuum evaporator , the method is not only extremely simple , but also remarkably reliable in routine use . Important considerations in establishing a standard procedure include ...
Page 458
... PROCEDURE Transport methods of gel filtration comprise the column procedure and the thin - layer technique , which differ only in practical facets of meth- odology such as the shape of the gel bed and the manner in which the mobility of ...
... PROCEDURE Transport methods of gel filtration comprise the column procedure and the thin - layer technique , which differ only in practical facets of meth- odology such as the shape of the gel bed and the manner in which the mobility of ...
Page 471
... procedure , and in none of the reported applications is it certain that the potential accuracy has been realized fully since exrapolation procedures were not generally employed . However , most of the gel filtration experiments were per ...
... procedure , and in none of the reported applications is it certain that the potential accuracy has been realized fully since exrapolation procedures were not generally employed . However , most of the gel filtration experiments were per ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone